Regulatory

Part:BBa_K1548001

Designed by: Michael Jacus   Group: iGEM14_Pitt   (2014-10-07)

Hsp60 promoter (M. bovis) and RBS (mycobacteriophage TM4)

Strong constitutive promoter from M. bovis fused to a strong RBS from TM4 bacteriophage. This hybrid promoter/RBS has been shown to function in a variety of mycobacteria. (1) It is expected to function in P. acnes a closely-related propionibacterium.

References: 1. Oldfield LM, Hatfull GF. Mutational Analysis of the Mycobacteriophage BPs Promoter PR Reveals Context-Dependent Sequences for Mycobacterial Gene Expression. J Bacteriol. 2014 Oct 15;196(20):3589-97. doi: 10.1128/JB.01801-14. Epub 2014 Aug 4. Sequence and Features Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 202
  • 1000
    COMPATIBLE WITH RFC[1000]


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